| The Change of Taurine in The Transient Global Ischemic Rabbit. |
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Sang Wook Shin, Hae Kyu Kim, Seong Wan Baik, Kyoung Seok Seo, Inn Se Kim, Kyoo Sub Chung |
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Department of Anesthesiology, College of Medicine, Pusan National University, Pusan, Korea. |
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| Abstract |
BACKGROUNDS
The importance of toxic extracellular levels of excitatory amino acids, as well as of high extracellular levels of inhibitory amino acids, is considered to be related with the pathophysiology of neuronal cell loss during cerebral ischemia.
METHODS
The extracellular concentration of glutamate, glycine, and taurine in the hippocampus were determined during and after transient global ischemia in the New Zealand white rabbit. Because the cerebroprotective effects of profound hypothermia are well known and clinically widely used,we used the hypothermia group as an experimental group compared with normothermic control group in the experimental protocol. To derive a quantitative descriptor reflecting the magnitude of amino acid neurotransmitter changes with ischemia, we defined the 'taurine index' as: [glutamate]x[glycine]/[taurine].
RESULTS
Ischemia led to large increases in both excitatory (glutamate and glycine) and inhibitory amino acids(taurine) in the normothermic group. Taurine index was increased during ischemia and after reperfusion and had a close relationship with the severity of ischemia-induced neuronal damage.
CONCLUSIONS
The taurine index appears to be a reliable biochemical marker to determine the severity of ischemic injury. |
| Key Words:
Animal rabbit; Brain hippocampus; ischemia; Hypothermia; Protein amino acid |
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