| The Effect of Intrathecal Bupivacaine with Hypothermia on Neuronal Protection against Transient Spinal Cord Ischemia in Rats. |
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Jeong Rak Lee, Jeong Gil Leem, Seung Jun Hwang, Dong Min Jang, Jung Won Kim |
1Department of Anesthesiology and Pain Medicine, College of Medicine, Inje university, Ilsan, Korea.
2Department of Anesthesiology and Pain Medicine, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea. jgleem@amc.seoul.kr
3Department of Anatomy, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea. |
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| Abstract |
BACKGROUND
Local anesthetics can reduece excitotoxic neuronal injury from ischemia. We investigated neuroprotective effects of intrathecally administered bupivacaine and hypothermia in rat model of transient spinal cord ischemia.
METHODS
A PE-10 intrathecal catheter was implanted into thirty six male Sprague-Dawley rats through L4-5 interlaminar space. Animals of normothermia (N) and hypothermia (H) groups were administered 15microliter of normal saline, and 15microliter of 0.5% bupivacaine for bupivacaine (B) and bupivacaine-hypothermia (BH) groups.
Transient spinal cord ischemia was induced by inflation of a 2 F Fogarty catheter placed into aortic arch for 12 minutes.
During ischemia, rectal temperature was maintained to 37.0+/-0.5 degrees C for N and B groups, 34.5 +/- 0.5degrees C for H and BH groups. Motor and sensory deficit score were assessed 2 and 24 hour after reperfusion. Lumbar spinal cords were harvested for histopathology, and for immunoreactivity of heat shock protein 70 (HSP70).
RESULTS
The motor and sensory deficit score of N and B group was significantly higher than H group (P < 0.05) and BH group (P < 0.05). There were also significant difference in the motor and sensory deficit score between H and BH group at 24 hr (P < 0.05). Neuronal cell death and immunoreactivity of HSP70 was frequently observed in the N and B groups, but not in the H and BH groups.
CONCLUSIONS
These results suggest that intrathecal bupivacaine did not provide neuroprotection during normothermic transient spinal cord ischemia in rats, but it can enhance neuroprotective effects of hypothermia. |
| Key Words:
bupivacaine; hypothermia; spinal cord ischemia |
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