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Korean J Anesthesiol > Volume 51(2); 2006 > Article
Korean Journal of Anesthesiology 2006;51(2):207-215.
DOI: https://doi.org/10.4097/kjae.2006.51.2.207   
The Effect of Intrathecal Bupivacaine with Hypothermia on Neuronal Protection against Transient Spinal Cord Ischemia in Rats.
Jeong Rak Lee, Jeong Gil Leem, Seung Jun Hwang, Dong Min Jang, Jung Won Kim
1Department of Anesthesiology and Pain Medicine, College of Medicine, Inje university, Ilsan, Korea.
2Department of Anesthesiology and Pain Medicine, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea. jgleem@amc.seoul.kr
3Department of Anatomy, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea.
Local anesthetics can reduece excitotoxic neuronal injury from ischemia. We investigated neuroprotective effects of intrathecally administered bupivacaine and hypothermia in rat model of transient spinal cord ischemia.
A PE-10 intrathecal catheter was implanted into thirty six male Sprague-Dawley rats through L4-5 interlaminar space. Animals of normothermia (N) and hypothermia (H) groups were administered 15microliter of normal saline, and 15microliter of 0.5% bupivacaine for bupivacaine (B) and bupivacaine-hypothermia (BH) groups. Transient spinal cord ischemia was induced by inflation of a 2 F Fogarty catheter placed into aortic arch for 12 minutes. During ischemia, rectal temperature was maintained to 37.0+/-0.5 degrees C for N and B groups, 34.5 +/- 0.5degrees C for H and BH groups. Motor and sensory deficit score were assessed 2 and 24 hour after reperfusion. Lumbar spinal cords were harvested for histopathology, and for immunoreactivity of heat shock protein 70 (HSP70).
The motor and sensory deficit score of N and B group was significantly higher than H group (P < 0.05) and BH group (P < 0.05). There were also significant difference in the motor and sensory deficit score between H and BH group at 24 hr (P < 0.05). Neuronal cell death and immunoreactivity of HSP70 was frequently observed in the N and B groups, but not in the H and BH groups.
These results suggest that intrathecal bupivacaine did not provide neuroprotection during normothermic transient spinal cord ischemia in rats, but it can enhance neuroprotective effects of hypothermia.
Key Words: bupivacaine; hypothermia; spinal cord ischemia
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