| The Role of Adrenergic and Cholinergic Receptors on the Antinociception of Intrathecal Zaprinast in the Formalin Test of Rats. |
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So Jeong Yoon, Yeo Ok Kim, Lan Ji Huang, Jin Hua Cui, Bong Hwa Heo, Sung Tae Jeong, Myung Ha Yoon |
1Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, Korea. mhyoon@chonnam.ac.kr
2Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju, Korea. |
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| Abstract |
BACKGROUND
Spinal zaprinast, phospodiesterase inhibitor, has been shown to have an antinociception through an increase of cGMP. The aim of this study was to examine the role of spinal adrenergic and cholinergic receptors on the antinociceptive action of intrathecal zaprinast.
METHODS
Rats were implanted with lumbar intrathecal catheters. After formalin injection, formalin-induced nociceptive behavior (flinching response) was observed for 60 min. After observing the effect of intrathecal zaprinast, antagonism of intrathecal prazosin, yohimbine, atropine and mecamylamine for the effect of zaprinast were evaluated.
RESULTS
Intrathecal zaprinast produced a dose-dependent suppression of formalin-induced flinches in both phases of the formalin test. Intrathecal prazosin reversed the antinociception of zaprinast in phase 2, but not phase 1.
Intrathecal yohimbine reversed the antinociception of zaprinast in both phases. Neither atropine nor mecamylamine reversed the antinocicetive action of zaprinast.
CONCLUSIONS
Intrathecal zaprinast is against the nociceptive state evoked by formalin stimulus. Alpha 2 or alpha 1 adrenergic receptor, but not cholinergic receptors, may be related to the action of zaprinast in the spinal cord. |
| Key Words:
adrenergic and cholinergic receptors; antinociception; formalin test; rat; spinal cord; zaprinast |
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