| The Influence of Propofol Concentration on the Generation of Reactive Oxygen Species after Reoxygenation in Rat Embryonic Heart H9c2 Cell. |
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Yun Hong Kim, Hyun Soo Kim, Young Jae Yi, Won Joon Choi, Jun Kyu Song, Seon Min Lee, Won Chae Choe, Sung Soo Kim |
1Department of Anesthesiology and Pain Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Korea. yhkim12@yahoo.co.kr
2Department of Molecular Biology, Kyung Hee University College of Medicine, Seoul, Korea. |
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| Abstract |
BACKGROUND
Reoxygenation of an ischemic heart causes a decrease in the cardiac function, which is known as reperfusion injury that is associated with an increase in the concentration of reactive oxygen species (ROS). This study examined the effect of the propofol concentration on the generation of ROS during reoxygenation in rat embryonic heart H9c2 cells.
METHODS
Cultured H9c2 cells were examined in the following sequences: Prehypoxic, Hypoxic and Reoxygenation period.
Each period required 60 minutes. The cells were exposed to propofol at the beginning of the prehypoxic period. Thirty minutes later, DCFH-DA (dichlorofluorescin diacetate) 10 micrometer was added to detect the ROS. The propofol concentrations used were 0, 5, 25, 50, 250 micrometer in the first experiment and 0, 1, 2, 3, 4, 5 micrometer in the second experiment. The ROS level was estimated using a fluorometer at 5-minute intervals from 5 to 60 minutes after reoxygenation.
RESULTS
When the propofol concentrations was > 5 micrometer, the ROS levels were significantly lower than those of the untreated group (P0) (P < 0.05). At propofol concentrations < 5 micrometer, the ROS levels 35 to 60 minutes after reoxygenation were significantly lower that in the untreated group (P < 0.05). Between 5 and 30 minutes after reoxygenation, the cells exposed to 1, 4 and 5 micrometer propofol also showed lower ROS levels than the untreated group P0. However, 2 and 3 micrometer propofol did not show any significant difference in ROS values to those observed in the untreated group except for 2 micrometer at 25 minutes after reoxygenation.
CONCLUSIONS
During the reoxygenation period in H9c2 cells, propofol concentrations > 5 micrometer inhibited ROS production over the whole period, and even 1micrometer showed some inhibition of ROS. |
| Key Words:
cells; heart; propofol; rats; reactive oxygen species; reperfusion |
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