| Do Microencapsulated Bovine Adrenal Medullary Chromaffin Cells Transplanted in Rats' Spinal Cord Produce Analgesic Effects? |
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Chi Hong Ahn, Young Hoon Jeon, Kyu Ho Lee, Guang Chun Jin, Yu Mi Kim, Jeong Ok Lim, Woon Yi Baek, Jin Woong Park |
1Department of Anesthesiology and Pain Medicine, School of Medicine, Kyungpook National University, Daegu, Korea. webaik@knu.ac.kr
2Department of Biomedical Engineering, School of Medicine, Kyungpook National University, Daegu, Korea.
3Medical Research Institute, School of Medicine, Kyungpook National University, Daegu, Korea. |
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| Abstract |
BACKGROUND
Pain remains the chief complaint that brings patients to physician's office, despite recent insights into underlying mechanism and the identification of potential new therapeutic targets. In recent years, however, with the development of molecular biology cell transplantation gives us a new chance for treating intractable chronic pain. The major purpose of the present study was to determine if the chromaffin cells that were encapsulated with 1.3% (w/v) sodium alginate-poly-l-lysine-alginate (APA) had robust analgesic effects in the spinal atlanto-occipital subarachnoid space even without nicotine stimulation.
METHODS
In order to determine whether microencapsulated bovine adrenal medullary chromaffin cells transplanted in the spinal cord can produce analgesic effects, we microencapsulated adrenal medullary chromaffin cells with APA and implanted them into the subarachnoid space of rats' (n = 10) spinal cord, and investigated the hot sensitivity of rats' hind-paw by a light-beam test.
RESULTS
It was found that compared with the control group, hot response latency of the group which received adrenal medullary chromaffin cells increased from the 12th day and the analgesic efficacy was maintained for at least 75 days.
CONCLUSIONS
Microencapsulated bovine adrenal medullary chromaffin cells transplanted in the rats' spinal cord may provide a permanent and locally available source of neuropeptides for the relief of intractable pain.
Furthermore, these kinds of analgesic effect were produced without any stimulation such as nicotine. |
| Key Words:
Analgesic effects; bovine adrenal medullary chromaffin cells; implantation; microencapsulation; rats spinal cord |
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