This article has been corrected. See "Effects of sevoflurane on neuronal cell damage after severe cerebral ischemia in rats" in Volume 73 on page 85.
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Effects of sevoflurane on neuronal cell damage after severe cerebral ischemia in rats
- Hee-Pyoung Park1, Eun-Ju Jeong1, Mi-Hyun Kim1, Jung-Won Hwang2, Young-Jin Lim1, Seong-Won Min3, Chong-Soo Kim3, Young-Tae Jeon2
- Received January 25, 2011 Revised May 17, 2011 Accepted May 21, 2011
- Abstract
-
- Background
- The aim of this study was to investigate the neuroprotective effects of sevoflurane after severe forebrain ischemic injury. We also examined the relationship between the duration of ischemia and neuronal cell death.
- Methods
- Male Sprague-Dawley rats (300-380 g) were subjected to 6 (each n = 6) or 10 min (each n = 10) of near-complete forebrain ischemia while anesthetized with either 50 mg/kg of zoletil given intraperitoneally or inhaled sevoflurane (2.3%). Ischemia was induced by bilateral common carotid artery occlusion plus hemorrhagic hypotension (26-30 mmHg). Histologic outcomes were measured 7 days after ischemia in CA1 pyramidal cells of the rat hippocampus.
- Results
- The mean percentage of necrotic cells in the hippocampal CA1 area decreased in the sevoflurane group compared to the zoletil group (25% vs. 40% after 6 min ischemia, respectively: P = 0.004 and 44% vs. 54% after 10 min of ischemia, respectively P = 0.03). The percentage of apoptotic cells was similar in all groups. The percentage of necrotic cells in each anesthetic groups was significantly higher in the 10 min ischemia group compared to the 6 min ischemia group (P = 0.004 in the sevoflurane group, P = 0.03 in the zoletil group).
- Conclusions
- The present data show that sevoflurane has neuroprotective effects in rats subjected to near-complete cerebral ischemia. Longer duration of ischemia is associated with more neuronal injury when compared to ischemia of shorter duration.
- Introduction
- Introduction
Numerous studies have demonstrated some degree of a neuroprotective effect with isoflurane [1,2], sevoflurane [3,4] and desflurane [2,5] after cerebral ischemia. The neuroprotective effects of anesthetic agents depend on the severity of ischemic injury. During less severe ischemia, anesthetic agents (fentanyl, ketamine, and isoflurane) resulted in no differences in outcome. In contrast, in animals sustaining a severe insult, those that were anesthetized with isoflurane had less damage than did rats given either ketamine or fentanyl [6].Zoletil is a combination of a dissociative anesthetic, tiletamine hydrochloride, and a benzodiazepine, zolazepam hypochloride. Tiletamine is a noncompetitive antagonist at the phencyclidine site of N-methyl-D-aspartate receptor.Sevoflurane has been shown to provide neuroprotection against focal [3] or incomplete global cerebral ischemia [4] in rats. To our knowledge, there has been no effort to define the effects of sevoflurane against severe forebrain global ischemia. The purpose of the present study was to determine the neuroprotective effects of sevoflurane on severe forebrain ischemic injury. We also examined the relationship between ischemic duration and neuronal death. Neuroprotection was assessed by histopathological evaluation 7 days after ischemia. Degrees of neuronal damage in ischemic hippocampal CA1 cells were assessed by counting necrotic cells after H&E staining and detection of DNA fragmentation was performed by terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling (TUNEL) staining.
- Materials and Methods
- Materials and Methods
The following study was approved by the Institutional Animal Care and Use Committee. Male Sprague-Dawley rats (300-380 g, 10-16 wks) were fasted 12-16 h before the experiments but were allowed free access to water. Rats were then randomly assigned to one of 4 conditions based on the anesthetics and duration of ischemia. In the 6 (n = 6) or 10 min (n = 10) sevoflurane group, the animals were anesthetized with 5% sevoflurane in oxygen and during surgery, a level of 2.3% sevoflurane in 100% O2 was maintained under spontaneous breathing. In the 6 (n = 6) or 10 min (n = 10) zoletil group, 50 mg/kg of zoletil (Virbac, Nice, France) was given intraperitoneally. The tail artery was catheterized with a PE-50 catheter to allow continuous recording of arterial blood pressure and blood sampling. The common carotid arteries were encircled with suture. The right jugular vein was cannulated with a silicone catheter for drug infusion and blood withdrawal. A 22-gauge needle thermistor was percutaneously placed adjacent to the skull beneath the temporalis muscle, and pericranial temperature was servoregulated (model TCAT-2 Temperature Controller; Harvard apparatus, Holliston, MA, USA) at 37.5 ± 0.1℃ by surface heating or cooling. Heparin (50 U) was given intravenously.Anesthetic conditions were established 30 min before ischemia, during which time rats in both groups were allowed to stabilize physiologically. Arterial blood gases and hemoglobin were measured 10 min before and after ischemia.Transient global ischemia was induced by bilateral common carotid artery occlusion and bleeding to lower the mean arterial pressure (MABP) to 26-30 mmHg using the method originally described by Smith et al. [7]. The carotids were then occluded with aneurysm clips. After 6 or 10 min of ischemia, the shed blood was reinfused. After regaining consciousness, the animals were maintained in an air conditioned room at 20℃. For each recovery interval, a set of sham rats was generated (n = 4). These animals were exposed to all aspects of sevoflurane anesthesia and surgical preparation for ischemia. The carotids were not occluded, and systemic hypotension was not used.After completion of the ischemic protocol, rats were allowed to recover for 7 days. All rats were anesthetized with sevoflurane, and the brains were fixed in situ by intraaortic infusion with buffered 10% formalin. Paraffin-embedded brain sections were serially cut (5 µm thick) and stained with hematoxylin and eosin. With the investigator blinded to group assignment, injury to the hippocampal CA1 sector was evaluated by microscopy. The total number of neurons (viable plus nonviable) was counted for each animal. The percentage of necrotic (eosinophilic) CA1 neurons was calculated as necrotic neurons/total neurons ×100. In each animal, five optical fields of the hippocampal CA1 region were examined.For the detection of DNA fragmentation, TUNEL staining was performed with the Apoptag® Peroxidase In Situ Apoptosis Detection Kit S7100 (Millipore Corporation, Billerica, USA). After deparaffinizing with xylene and graded concentrations of alcohol, brain sections were exposed to Proteinase K for 15 min at room temperature. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in phosphate buffered saline (PBS) for 5 min at room temperature. Sections were then incubated with terminal deoxynucleotidyl transferase (TdT) in a humidified chamber at 37℃ for 1h. After incubation with anti-digoxigenin-conjugate for 30 min at room temperature, peroxidase substrate (0.05% diaminobenzidine, DAB) was applied to develop color. The specimens were then washed with distilled water and were counterstained with 0.5% methyl green for 10 min at room temperature. TUNEL-positive neurons that contained apoptotic bodies were identified as being neurons undergoing apoptosis. The number of apoptotic cells was counted under high-power microscopic magnification (×400). In each animal, five optical fields of the hippocampal CA1 region were examined. The percentage of TUNEL-positive cells was defined as the percentage of the number of TUNEL-positive cells to the total cell number.The quantitative data were presented as the mean ± SD. Physiologic parameters were analyzed by repeated measures analysis of variance. Statistical significance between groups was analyzed using a Mann-Whitney U test. Differences were considered significant when P < 0.05.
- Results
- Results
Physiologic parameters are summarized in Table 1. There were no significant differences in mean arterial pressure, pH, PaCO2, PaO2, and hemoglobin concentration between the control and the sevoflurane treated groups at any time point.Of the 20 animals receiving 10 min of ischemia, five rats in the zoletil treated group died before the determination of neurologic damage. A majority of the postoperative deaths occurred within 3 days of ischemia. No animals died after 6 min of ischemia and no neuronal death was observed in the sham-operated group. The mean percentage of necrotic cells in the zoletil 6 min group was significantly higher than in the sevoflurane 6 min group (Fig. 1: P = 0.004) and similar results were seen in the zoletil 10 and sevoflurane 10 group (Fig. 2: P = 0.03). The percentage of necrotic cells in each anesthetic group was significantly higher in the 10 min ischemia group compared to the 6 min ischemia group (Fig. 1, Fig. 2: P = 0.004 in the sevoflurane group, P = 0.03 in the zoletil group).
- Discussion
- Discussion
In the present study, we investigated the neuroprotective effect of sevoflurane on near-complete cerebral ischemia. This study demonstrated that histologic outcome after 6 or 10 min of severe forebrain ischemia were substantially better in rats with sevoflurane versus zoletil anesthesia 7 days after ischemia. Sevoflurane did not decrease ischemia-induced apoptotic cell death 7 days after ischemia. Shorter duration of ischemia was associated with less neuronal damage.In rats subjected to incomplete cerebral ischemia, sevoflurane improved neurologic outcomes [4]. In the present study, we focused on the severity of cerebral ischemia. Others have shown that there was no effect of anesthetic agents on outcome in less severe incomplete ischemia [6]. Cerebral blood flow studies after the near-complete insult causing EEG isoelectricity have consistently found flow to be severely reduced. In contrast, examination of cerebral blood flow during less severe forebrain injury found substantial scatter in the magnitude of cerebral blood flow reduction [8]. The near-complete ischemia insult was uniformly severe across all animals. In contrast, findings in the incomplete model varied with respect to concordance between cortical and hippocampal regions as well as between the presence or absence of EEG activity [6]. In the current study, sevoflurane showed better histologic outcomes in rats subjected to severe cerebral ischemia. Six min of ischemia also induced severe neuronal injury. In contrast to our study, a previous study showed sevoflurane completely prevented the neuronal eosinophilic damage (a valid indicator of necrosis) of the brain in incomplete cerebral ischemia [9]. The neuroprotective effect of anesthetic agents seems to be correlated with the severity of neurologic injury. Anesthetic agents provide sustained neuroprotection in the presence of less severe ischemic states [9,10]. In contrast, in a rat focal ischemia model, isoflurane did not lead to sustained neuroprotection after 14 days [11]. This suggests that the severity of cerebral ischemia is beyond the neuroprotective potential of anesthetic agents to prevent necrotic cell death.The mechanisms by which volatile anesthetic agents potentially protect the brain are still unclear. Cerebral protection with sevoflurane has been explained by a reduction in cerebral requirements, similar to barbiturates or isoflurane. The neuroprotective effects of volatile anesthetic agents are associated with decreased apoptotic cell death in the postischemic period [12-15]. Anesthesia with sevoflurane induces a sustained inhibition of neuronal injury by promoting anti-apoptotic pathways [9,16]. It was demonstrated that sevoflurane prevents the ischemia-induced increase of the pro-apoptotic protein Bax 4 h after injury induced by incomplete cerebral ischemia [16]. Sevoflurane increases the hippocampal concentration of the anti-apoptotic proteins Bcl-2 and Mdm2 in addition to inhibiting the upregulation of Bax [9]. In the present study, sevoflurane did not decrease TUNEL positive cells. Kawaguchi et al.[13] showed that volatile anesthetics delayed but did not prevent neuronal apoptosis after focal cerebral ischemia. We propose that any anti-apoptotic effect of sevoflurane might disappear 7 days after ischemia.In the present study, shorter durations of ischemia had significantly less damage than longer durations (i.e. 6 vs 10 min). Consistent with our study, the magnitude of ischemic injury is dependent on the duration of ischemia [17]. Sevoflurane did not prevent neuronal damage following ischemia of 6 min duration. We speculate that even short durations of severe hypotension can cause severe damage which cannot be prevented by sevoflurane.We used 2.3 vol% sevoflurane for maintenance of anesthesia. One minimal alveolar concentration of sevoflurane is 2.3 vol% in adult rats [18]. Therefore, the sevoflurane concentration used in this study was relevant.There are several limitations in this study. First, the study was limited to 7 days after ischemia. Longer periods of time may be necessary to assess the permanent neuroprotective effect of anesthetics. However, maintaining rats for such a long period of time after cerebral ischemia is not feasible in our laboratory. Second, we did not confirm EEG isoelectricity. However, it is well demonstrated that bilateral common carotid artery occlusion plus hemorrhage (30 mmHg) induces near-complete ischemia in the CA1 region of rats [6,19]. Third, we classified necrosis and apoptosis. Attempts to classify CA1 neuronal death as either necrosis or apoptosis may be misleading when attempting to understand mechanisms of death of differentiated neurons. These neurons might possess some of the phenotypic characteristics of necrotic neurons but, in contrast, possess all of the biochemical and molecular characteristics needed to die via apoptosis.In summary, this study shows that sevoflurane improves neurological outcome after severe cerebral ischemia in rats compared with animals anesthetized with zoletil. Longer duration of cerebral ischemia is associated with more neuronal damage than shorter durations of ischemia.
- Acknowledgments
Supported by grant to 04-2007-002 from the SNUBH Research Fund.
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Fig. 1
The percentage of necrotic and apoptotic cells in the hippocampus 7 days after forebrain ischemia. The percentage of necrotic cells was lower in the sevoflurane 6 min ischemia group (*P < 0.05). There is no significant difference between groups with respect to apoptotic cell number. All data are expressed as mean ± SD.
Fig. 2
The percentage of necrotic and apoptotic cells in the hippocampus 7 days after forebrain ischemia. The percentage of necrotic cells was lower in the sevoflurane 10 min ischemia group (*P < 0.05). The percentage of necrotic cells in each anesthetic group is higher in the 10 min ischemia group compared with the 6 min ischemia group (†P < 0.05). There are no significant differences between groups with respect to apoptotic cell numbers. All data are expressed as mean ± SD.
