| Effect of vasopressin on survival of Purkinje neurons in rat cerebellar slices after an in vitro simulated ischemia. |
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Yun Seok Jeon, Soo Young Lee, Jiwon Lee, Nam Su Gil, Young Jin Lim, Yong Chul Kim, Sang Chul Lee |
1Department of Anesthesiology and Pain Medicine, College of Medicine, Seoul National University, Seoul, Korea. limyjin@snu.ac.kr
2Department of Anesthesiology and Pain Medicine, Boramae Municipal Hospital, Seoul, Korea. |
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| Abstract |
BACKGROUND
Arginine vasopressin (AVP) is frequently used in patients under the risk of brain injury. It has been shown to induce brain injury after ischemia and reperfusion in in vivo animal models. We determined the effect of vasopressin on the brain injury after ischemia-reperfusion using in vitro model.
METHODS
Cerebellar brain slices were prepared from adult Sprague-Dawley rats. They were then subjected to simulated ischemia (oxygen-glucose deprivation) for 20 min in the absence (control) or presence of vasopressin (5, 10, 50, 100, 500 pg/ml). After being recovered in oxygenated artificial cerebrospinal fluid for 5 h, they were fixed for morphologic examination to determine the percentage of live Purkinje cells.
RESULTS
There were no differences in the survival rate of Purkinje cells among the control and vasopressin groups.
CONCLUSIONS
Vasopressin at concentrations studied has no direct effect on brain ischemia-reperfusion injury. |
| Key Words:
Brain; Ischemia; Rat; Vasopressin |
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