| Effect of ginsenosides on the desflurane modulation in the recombinant serotonin type 3A receptor expressed in Xenopus laevis oocytes. |
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Seung Ho Choi, Mi Kyeong Kim, Bon Nyeo Koo, Kyeong Tae Min |
1Department of Anesthesiology and Pain Medicine, Yonsei University Health System, Seoul, Korea. ktmin501@yuhs.ac
2Research Institute of Anesthesia and Pain, Yonsei University, Seoul, Korea.
3Department of Anesthesiology and Pain Medicine, Kyung Hee University College of Medicine, Seoul, Korea. |
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| Abstract |
BACKGROUND
Postoperative nausea and vomiting (PONV) is the most frequent and discomforting side effect following general anesthesia. Most volatile anesthetics have a potent effect on serotonin (5-hydroxydtryptamine, 5-HT) type 3 receptor mediating PONV, and their antagonists have been currently used effectively to prevent and/or reduce the incidence and severity of PONV. The authors reported previously that ginsenosides have inhibitory effect on 5-HT3A receptor. In this study we intended to elucidate the inhibitory effect of ginsenosides on the potentiated 5-HT3A receptor by desflurane.
METHODS
After in vitro transcription of the recombinant mouse 5-HT3A receptor in the Xenopus laevis oocyte, we examined the effects of ginsenosides (g-Rb1, g-Rg1, g-Rd, g-Rg2) as well as ginsenoside metabolite, compound K on the modulation of desflurane by measuring currents flowing through 5-HT3A receptor using two-electrode voltage clamp technique.
RESULTS
Although normalized inhibitory responses of ginsenosides were same regardless of desflurane, some ginsenosides such as g-Rd, g-Rg2, and g-Rg1 showed potential inhibition to the enhanced 5-HT induced current of 5-HT3A receptor by desflurane.
CONCLUSIONS
Although ginsenosides have substantial inhibitory effect on 5-HT3A receptor, the effects of ginsenoside on potentiation by desflurane of 5-HT induced current via recombinant 5HT3A receptor may depend on the types of ginsenoside, which suggesting that ginsenoside might have an antagonistic action to nausea and vomiting associated with volatile anesthetics. |
| Key Words:
Desflurane; Ginsenosides; Serotonin type 3A receptor; Xenopus laevis oocyte |
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