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Korean J Anesthesiol > Volume 54(3); 2008 > Article
Korean Journal of Anesthesiology 2008;54(3):328-334.
DOI: https://doi.org/10.4097/kjae.2008.54.3.328   
Expression of Spinal c-fos in a Rat Model of Postoperative Pain.
Sok Ju Doh, Suk Ki Choi, Hee Cheol Jin
1Graduate School, College of Medicine, Soonchunhyang University, Korea.
2Department of Anesthesiology and Pain Medicine, Soonchunhyang University Hospital, Bucheon, Korea. anecl0503@yahoo.co.kr
Abstract
BACKGROUND
The oncogene, c-fos, is a useful marker of nociceptive neurons activated by various types of pain.However, the relationship between c-fos and acute pain induced by surgical incision has not been studied.The purpose of this study was to describe the expression of spinal c-fos in a rat model of postoperative pain.
METHODS
Sprague-Dawley rats were used to enumerate spinal fos-like immunoreactive (fos-LI)-positive neurons after making sham, skin, or skin-muscle incisions on the plantar surface of the rat hindpaw. The spinal cords were divided into the following 4 segments for immunohistochemical detection of fos-LI at 3 time points after the incision (4 hours, POD1, and POD3):thoracic, upper lumbar, lower lumbar, and sacral.For analysis of the laminar distribution of fos-LI, the dorsal horn was divided into three regions (I-II, III-IV, and V-VI).
RESULTS
The number of fos-LI was increased in the ipsilateral spinal dorsal horn in the lower lumbar segment 4 hours post-incision and was significantly greater in rats with skin-muscle incisions than in rats with skin-alone incisions.In the skin-muscle group, laminae I-II and V-VI had a greater number of fos-LI neurons than laminae III-IV.The increase in number of spinal fos-LI neurons normalized by POD1 and POD3.
CONCLUSIONS
c-fos expression is confined to neurons of the spinal dorsal horn known to be activated by nociceptive stimulation. The larger the injury elicited, the greater the expression of c-fos, suggesting that the expression of c-fos can serve as a useful marker of activated nociceptive neurons caused by acute pain following surgical incision.Further study is required to elucidate why the degree of tissue injury affects the laminar distribution of c-fos expression.
Key Words: c-fos; postoperative pain; spinal


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