Korean J Anesthesiol Search


Korean Journal of Anesthesiology 2000;38(5):894-903.
DOI: https://doi.org/10.4097/kjae.2000.38.5.894   
The Effects of Transection Injury and Local Infiltration of Phenol at the Sciatic Nerve on c-fos Expression in the Spinal Cords of Rats.
Hyun Kyung Lim, Chong Kweon Chung, Tae Jung Kim, Jae Kue Shin, Young Deog Cha, Dong Ho Park, Ik Sang Seung, Jeong Uk Han
1Department of Anesthesiology, College of Medcine, Inha University, Incheon, Korea.
2Department of Anesthesiology, College of Medcine, Hanyang University, Seoul, Korea.
Phenol has effects like surgical neurectomy, but may evoke pain after local infiltration in nerves. Transection of peripheral nerves may induce neuropathic pain through increased spontaneous discharge and other mechanisms. Proto-oncogene, c-fos, is an indicator of neuronal activity, and its expression in the spinal cord may be related to pain development, because inhibition of c-fos expression has corresponding effects like analgesia. We evaluated the effects of local infiltration of phenol and transection injury at the sciatic nerve on c-fos expression in the spinal cords of rats.
Sixteen male Sprague-Dawley rats were divided into 2 groups; transection of the sciatic nerve was performed for group 1; phenol was infiltrated into the sciatic nerve in group 2. Three hours, 1 week, 2 weeks, and 3 weeks after the experiment, the corresponding spinal cord was stained immunohistochemically for c-fos.
c-fos was expressed from 3 hours to 2 weeks over the laminae of the dorsal horn in each group. Phenol increased the expression of c-fos initially, but decreased 1 week later. Transection injury did not increase it initially, but showed the peak expression at 1 week, and maintained it for 2 weeks. Therefore, it seems that phenol, rather than the transection injury, stimulates c-fos expression early, but decreases later.
Phenol treatment, caused by chemical block due to protein denaturation and nonspecific inflammation, may induce less neuropathic pain than the transection of a nerve.
Key Words: Spinal cord: c-fos; laminae of dorsal horn; Toxicity: phenol


Browse all articles >

Editorial Office
101-3503, Lotte Castle President, 109 Mapo-daero, Mapo-gu, Seoul 04146, Korea
Tel: +82-2-792-5128    Fax: +82-2-792-4089    E-mail: journal@anesthesia.or.kr                

Copyright © 2024 by Korean Society of Anesthesiologists.

Developed in M2PI

Close layer
prev next