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Korean Journal of Anesthesiology 1998;34(5):910-914.
DOI: https://doi.org/10.4097/kjae.1998.34.5.910   
Influence of Decamethonium on the Neuromuscular Blocking Action of d-Tubocurarine in the Rat Phrenic Nerve Hemidiaphragm Preparation.
Kyung Ho Hwang, Joo Eun Song, Sun Hak Lee, Jeong Seok Lee, Wook Park, Sung Yell Kim
Department of Anesthesiology, College of Medicine, Soon Chun Hyang University, Seoul, Korea.
To elucidate the mechanism of interaction between depolarizing and nondepolarizing muscle relaxants, train-of-four (TOF) fade during onset of neuromuscular blockade of d-tubocurarine (dTC) with or without decamethonium (C10) was evaluated in a rat phrenic nerve hemidiaphragm preparation.
Phrenic nerve hemidiaphragm preparations from 250~300 g Sprague Dawley rats (n=20) were suspended in a Krebs solution bubbled with 5% CO2 in O2 at 32oC. Phrenic nerves were stimulated with supramaximal stimuli of 0.2 ms duration at 0.15 Hz single twitch and 2 Hz TOF by a Grass S88 stimulator and the contractions of the hemidiaphragm were detected by a Grass FT03 force transducer then recorded. Estimation of ED50 for the dose response data were performed by a linear regression. The statistical significance of the results was determined by Wilcoxon Rank Sum test. p<0.05 was considered significant.
Mean ED50 values of dTC and C10 calculated from the dose response relations were 7.76 microgram/ml and 0.65 microgram/ml respectively. Compared to adminstration of 2xED50 of dTC alone, TOF ratios at 75% and 50% of twitch height were markedly decreased by combination of ED50 of C10 and ED50 of dTC with statistic significance (67 +/- 1.9% vs. 46 +/- 3.1% and 36 +/- 2.5% vs. 7 +/- 2.5%).
If fade in response to TOF stimulation represents a prejunctional effect, the results from this study suggests that the presynaptic action of C10 has some role in the mechanism of the interaction between dTC and C10 in the rat.
Key Words: Neuromuscular relaxants: d-tubocurarine; decamethonium; Pharmacology: drug interaction


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